Future studies should aim to evaluate neutralizing IgM responses to identify the origin of the B cell subsets that are responsible for their production

Future studies should aim to evaluate neutralizing IgM responses to identify the origin of the B cell subsets that are responsible for their production. linear mixed effect model using a Tukey-Kramer HSD post-hoc analysis. Asterisks show a infections. Kinetics of total IgM (a) and IgG (b) at different contamination stages during initial infections and relapses as determined by ELISA. (c) IgG subclasses realizing iRBC lysates as determined by ELISAs. The black line indicates background. Pink diamonds = malaria na?ve, orange circles = initial infections, and green triangles = relapse infections. Bars show the mean of the data points shown; Error Bars = SEM. Statistical significance was assessed by a linear mixed effect model using a Tukey-Kramer HSD post-hoc analysis. Asterisks show a p-value 0.05. All ELISAs were repeated two times.(TIF) ppat.1007974.s006.tif (899K) GUID:?4B79730F-401F-47B6-8BAD-8C7F659E31F5 S7 Fig: Anti-uRBC IgG and IgM are inversely correlated with hemoglobin levels during primary infections. Anti-uRBC and anti-iRBC antibody response for IgM (a) and IgG (b) during main infections and relapses as determined by ELISA. Dashed and solid lines show background levels as defined by the mean of the malaria na? ve samples plus three standard deviations for uninfected and infected RBCs, respectively. Spearmans correlation analysis of anti-uRBC IgM (c) and anti-uRBC IgG (d) with hemoglobin levels during main and relapse infections. Bars show the mean of the data points shown; Error Bars = SEM. Statistical significance was assessed by a linear mixed effect model using a Tukey-Kramer HSD post-hoc analysis. Asterisks show a p-value 0.05. All ELISAs were repeated two times.(TIF) ppat.1007974.s007.tif (955K) GUID:?728E24A5-05A8-4342-86E2-8CF256A75285 S8 Fig: Pre-existing immunity does not change day-to-patency during homologous challenges. The days to patency for the initial infections and homologous difficulties are shown. Statistical significance was assessed by a Wilcoxon test.(TIF) ppat.1007974.s008.tif (179K) GUID:?6FCBA783-886C-4B0C-9F1F-9C2DD1A31931 S9 Fig: Hemoglobin and platelet kinetics with parasitemia during homologous GSK1059865 challenges with M/B strain in rhesus macaques. Daily hemoglobin levels (a) and platelet figures (b) during homologous difficulties GSK1059865 with M/B strain. The five-letter code around the left-hand side of each graph indicates a different individual. k = multiply number shown by 1,000.(TIF) ppat.1007974.s009.tif (1.2M) GUID:?68E81214-8558-4A00-9CB2-723FEEFC8361 S10 Fig: Spearmans correlation analysis of the relationship between na?ve and double unfavorable B cells during relapses and homologous reinfections. = Spearmans correlation coefficient.(TIF) ppat.1007974.s010.tif (764K) GUID:?EA11EBD1-3DF3-484C-BD15-A28AE7301B64 S11 Fig: Total IgM, total IgG, and IgG subclass kinetics during relapses and homologous reinfections. Kinetics of total IgM (a) and IgG (b) at different contamination stages during relapses and homologous reinfections as determined by ELISA. (c) IgG subclasses realizing iRBC lysates as determined by ELISAs. The black line indicates the background. Pink diamonds = malaria na?ve, orange circles = initial infections, and green triangles = relapse infections. Bars show the mean of the data points shown; Error Bars GSK1059865 = SEM. Statistical significance was assessed by a linear mixed effect model TSHR using a Tukey-Kramer HSD post-hoc analysis. Asterisks show a p-value 0.05. All ELISAs were repeated two times.(TIF) ppat.1007974.s011.tif (940K) GUID:?929EE671-1B6A-464C-91E4-3B493587BA2B S12 Fig: Anti-uRBC IgM and IgG kinetics during relapses and homologous reinfections. Anti-uRBC and anti-iRBC antibody response for IgM (a) and IgG (b) during relapses and homologous reinfections as determined by ELISA. Dashed and solid lines show background levels show a true positive as defined by the mean of the malaria na?ve samples plus three standard deviations for uninfected and infected RBCs, respectively. Bars show the mean of the data points shown; Error Bars = SEM. Statistical significance was assessed by a linear mixed effect model using a Tukey-Kramer HSD post-hoc analysis. Asterisks show a.